Determination of catecholamines with lucigenin chemiluminescence.

Lucigenin chemiluminescence assay for superoxide detection.

Superoxide Detection To the Editor: The recent review by Tarpey and Fridovich published in Circulation Research1 mostly contains interesting and sound material; however, I would like to comment on the section entitled, “Superoxide Detection, Chemiluminescence Reactions.” Basing mainly on their own data,2 authors maintain that lucigenin-amplified chemiluminescence (LucCL) overestimates the rate ...

Investigation of alveolar macrophage function using lucigenin-dependent chemiluminescence.

The method using lucigenin-dependent phagocytic chemiluminescence is described for the assessment of alveolar macrophage metabolic activity in response to stimulation by opsonised particles or soluble agents. The requirement for superoxide anion (O2-) in the production of chemiluminescence is suggested by inhibition (95%) using superoxide dismutase. The results obtained are correlated with thos...

Automatic semi-microcolumn liquid chromatographic determination of catecholamines in rat plasma utilizing peroxyoxalate chemiluminescence reaction.

A fully automated and highly sensitive method with a semi-microcolumn liquid chromatography system for the determination of rat plasma catecholamines (CAs) was developed. Automated on-line extraction of CAs in diluted plasma using a precolumn packed with strong acidic cation exchange resin was coupled with separation of CAs on a semi-microcolumn (250 x 1.5 mm id). fluorogenic derivatization wit...

Determination of polyphenols with HPLC-sensitized chemiluminescence.

Pattern of formylmethionyl-leucyl-phenylalanine-induced luminol- and lucigenin-dependent chemiluminescence in human neutrophils.

The stimulation of neutrophils by formylmethionyl-leucyl-phenylalanine results in a bimodal luminol-dependent chemiluminescence pattern. We observed, however, only a single peak chemiluminescence pattern in the response to the peptide when we used lucigenin as an amplifying substance. We suggest that lucigenin, the larger molecule, (510 daltons; luminol is 177 daltons) only exerts an extracellu...